PE/Cyanine7 anti-Bcl-2 Antibody
- 产品名称：
- PE/Cyanine7 anti-Bcl-2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 633511
- 产品应用：
- 633511
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- N-terminal, Amino acid residues 61-76 of mouse Bcl-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
- Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
- Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
- Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
- Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
- Product Citations
-
- Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
- Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed
- Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- RRID
- AB_2565246 (BioLegend Cat. No. 633511) AB_2565247 (BioLegend Cat. No. 633512)
- Structure
- Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
- Distribution
-
Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
- Function
- Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
- Modification
- Phosphorylation, Ubiquitination
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
-
1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.
- Regulation
- Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
- Gene ID
- 596 View all products for this Gene ID
- UniProt
- View information about Bcl-2 on UniProt.org
