FITC anti-Bcl-2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1272	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

N-terminal, Amino acid residues 61-76 of mouse Bcl-2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue⁴, immunocytochemical staining⁵, and immunoprecipitation⁵.

Application References (PubMed link indicates BioLegend citation)

Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)

Product Citations

Yasuda K, et al. 2019. Nat Commun. 10:549. PubMed
Gautam S, et al. 2019. Nat Immunol. 20:337. PubMed
Todorov H, et al. 2022. iScience. 25:104927. PubMed
Mueller-Ortiz S, et al. 2014. J Immunol. 193:1278. PubMed
Crauste F, et al. 2017. Cell Syst. 0.379166667. PubMed
Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed

RRID

AB_2028392 (BioLegend Cat. No. 633503) AB_2028394 (BioLegend Cat. No. 633504)

Antigen Details

Structure: Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution: Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
Function: Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Modification: Phosphorylation, Ubiquitination
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.
Regulation: Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Gene ID: 596 View all products for this Gene ID
UniProt: View information about Bcl-2 on UniProt.org

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