Direct-Blot™ HRP anti-Bcl-2 Antibody
- 产品名称：
- Direct-Blot™ HRP anti-Bcl-2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 633513
- 产品应用：
- 633513
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- N-terminal, Amino acid residues 61-76 of mouse Bcl-2
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 ?g per mL (1:100 - 1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.
- Application References
(PubMed link indicates BioLegend citation) -
- Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
- Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
- Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
- Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
- Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
- RRID
- AB_2750245 (BioLegend Cat. No. 633513) AB_2750246 (BioLegend Cat. No. 633514)
- Structure
- Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
- Distribution
-
Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
- Function
- Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
- Modification
- Phosphorylation, Ubiquitination
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
-
1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.
- Regulation
- Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
- Gene ID
- 596 View all products for this Gene ID
- UniProt
- View information about Bcl-2 on UniProt.org
