Alexa Fluor® 647 anti-Bcl-2 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-Bcl-2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 633509
- 产品应用：
- 633509
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- N-terminal, Amino acid residues 61-76 of mouse Bcl-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.
- Application References
(PubMed link indicates BioLegend citation) -
- Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
- Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
- Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
- Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
- Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
- Product Citations
-
- Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
- Bauché D et al. 2018. Immunity. 49(2):342-352 . PubMed
- Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
- Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
- Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
- Beus J, et al. 2013. PLoS One. 8:71221. PubMed
- Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
- Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
- Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
- Severance AL, et al. 2022. iScience. 25:104400. PubMed
- Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed
- Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
- Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- Wang H, et al. 2016. PLoS Pathog. 12: 1005787. PubMed
- Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
- RRID
- AB_2064149 (BioLegend Cat. No. 633509) AB_2274702 (BioLegend Cat. No. 633510)
- Structure
- Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
- Distribution
-
Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
- Function
- Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
- Modification
- Phosphorylation, Ubiquitination
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
-
1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.
- Regulation
- Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
- Gene ID
- 596 View all products for this Gene ID
- UniProt
- View information about Bcl-2 on UniProt.org
