PerCP/Cyanine5.5 anti-human CD25 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302625
- 产品应用：
- 302625
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications include: immunocytochemistry3.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
- Product Citations
-
- Zhang Y, et al. 2022. Front Immunol. 12:799206. PubMed
- Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
- Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
- Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
- Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed
- Garcia EG, et al. 2020. Leukemia. 35:679. PubMed
- RRID
- AB_2125479 (BioLegend Cat. No. 302625) AB_2125478 (BioLegend Cat. No. 302626)
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
