Pacific Blue™ anti-human CD25 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302620
- 产品应用：
- 302620
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- test size: lot-specific; ?g sizes: 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 ?l per 106 cells in 100 ?l volume.
For ?g sizes, the suggested use of this reagent for immunofluorescent staining is ≤1.0 ?g per 106 cells in 100 ?l volume.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications include: immunocytochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
- Product Citations
-
- Olwenyi OA, et al. 2020. Cells. 9:. PubMed
- Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed
- Jager P, et al. 2009. Proc Natl Acad Sci U S A. 106:5264. PubMed
- Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed
- Blackburn M, et al. 2015. J Immunol. 195: 3227 - 3236. PubMed
- RRID
- AB_493649 (BioLegend Cat. No. 302620) AB_2233706 (BioLegend Cat. No. 302627)
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
