Brilliant Violet 650™ anti-human CD25 Antibo
- 产品名称：
- Brilliant Violet 650™ anti-human CD25 Antibo
- 产品类别：
- 抗体
- 产品编号：
- 302633
- 产品应用：
- 302633
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2844 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications include: immunocytochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
- Product Citations
-
- Lindesmith LC, et al. 2020. Cell Mol Gastroenterol Hepatol. 0.586805556. PubMed
- Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Bradley T, et al. 2020. Nat Commun. 11:948. PubMed
- Han Q, et al. 2020. Cell Rep. 30:1553. PubMed
- Riese P, et al. 2022. Nat Commun. 13:6894. PubMed
- Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
- Trivedi S, et al. 2020. Elife. 9:00. PubMed
- RRID
- AB_11203536 (BioLegend Cat. No. 302633) AB_2563807 (BioLegend Cat. No. 302634)
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
