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Brilliant Violet 421™ anti-human CD25 Antibo
产品名称:
Brilliant Violet 421™ anti-human CD25 Antibo
产品类别:
抗体
产品编号:
302629
产品应用:
302629
[价格]
规格 价格 库存
25tests ¥ 2220 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  2. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  3. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  4. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  5. Nesspor TC, et al. 2020. Sci Rep. 10:7557. PubMed
  6. Huang C, et al. 2020. Theranostics. 10:10498. PubMed
  7. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  8. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  9. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  10. Lam AJ, et al. 2021. Cell Reports. 36(5):109494. PubMed
  11. Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed
  12. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  13. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  14. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  15. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  16. Nowak A, et al. 2018. Front Immunol. 0.513194444. PubMed
  17. Gao X, et al. 2022. iScience. 25:104911. PubMed
  18. Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed
  19. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  20. Nakachi S, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1309-x. PubMed
  21. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  22. Hernndez DC, et al. 2021. Immunity. :. PubMed
  23. Pandya J, et al. 2016. J Leukoc Biol. 100: 823 - 833. PubMed
  24. Manuszak C, et al. 2020. J Biol Methods. 7:e131. PubMed
  25. Li H, et al. 2020. Front Oncol. 1.477083333. PubMed
  26. Nowatzky J, et al. 2020. J Vis Exp. . PubMed
  27. Fu J, et al. 2021. J Clin Invest. 131:. PubMed
  28. Lin-Shiao E, et al. 2022. Nucleic Acids Res. 50:1256. PubMed
RRID
AB_10896914 (BioLegend Cat. No. 302629) AB_11126749 (BioLegend Cat. No. 302630)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

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