Alexa Fluor® 700 anti-human CD25 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302621
- 产品应用：
- 302621
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications include: immunocytochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
- Product Citations
-
- Fang L, et al. 2013. Neuro Oncology. 15:1479. PubMed
- Wan Q, et al. 2011. J Exp Med. 208:1875. PubMed
- Wang J, et al. 2009. J Immunol. 183:4119. PubMed
- Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
- Andersch L, et al. 2019. BMC Cancer. 19:895. PubMed
- Rankin CR, et al. 2020. Am J Physiol Gastrointest Liver Physiol. 318:G34. PubMed
- Harper JL, et al. 2020. Nat Med. 519:26. PubMed
- Milliken S, et al. 2012. J Allergy Clin Immunol. 129:1554. PubMed
- Cornwell WD, et al. 2018. BMC Pulm Med. 0.820138889. PubMed
- RRID
- AB_493754 (BioLegend Cat. No. 302621) AB_493755 (BioLegend Cat. No. 302622)
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
