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Alexa Fluor® 488 anti-human CD25 Antibody
产品名称:
Alexa Fluor® 488 anti-human CD25 Antibody
产品类别:
抗体
产品编号:
302615
产品应用:
302615
[价格]
规格 价格 库存
25tests ¥ 1488 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Le Gall C, et al. 2022. J Immunother Cancer. 10:. PubMed
  2. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  3. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  4. Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed
  5. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  6. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  7. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  8. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  9. Baratella M, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005285. PubMed
  10. Pais H, et al. 2019. Sci Rep. 9:5760. PubMed
RRID
AB_493044 (BioLegend Cat. No. 302615) AB_493043 (BioLegend Cat. No. 302616)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

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