Ultra-LEAF™ Purified anti-mouse CD200R3 Anti
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD200R3 Anti
- 产品类别：
- 抗体
- 产品编号：
- 142215
- 产品应用：
- 142215
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse primary basophils
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Activ - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Ba13 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin.?Additional reported applications (for the relevant formats) include: stimulation of bone marrow derived basophils to produce IL-4.
- RRID
- AB_2888753 (BioLegend Cat. No. 142215) AB_2888753 (BioLegend Cat. No. 142216)
- Structure
- Immunoglobulin superfamily; disulfide-linked dimer with positively charge amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced conditions, respectively)
- Distribution
-
Basophils in peripheral blood, bone marrow, spleen; mast cells in peritoneal cavity and skin
- Function
- Transduce stimulatory signals
- Interaction
- DAP12, DAP10, FcRγ, or CD3ζ
- Cell Type
- Basophils, Mast cells
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117.
2. Kojima T, et al. 2007. J. Immunol. 179:7093.
3. Sato K, et al. 2009. Blood 113:4780.
- Gene ID
- 74603 View all products for this Gene ID
- UniProt
- View information about CD200R3 on UniProt.org
