Alexa Fluor® 647 anti-mouse CD366 (Tim-3) Anti
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD366 (Tim-3) Anti
- 产品类别：
- 抗体
- 产品编号：
- 134005
- 产品应用：
- 134005
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- mTim-3 protein/Freund adjuvant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone B8.2C12 only binds to the BALB/c allele of Tim-3.
- Application References
(PubMed link indicates BioLegend citation) -
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
-
- Mizukami S, et al. 2015. Proc Natl Acad Sci U S A. 112:1809. PubMed
- Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
- Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
- Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed
- RRID
- AB_1626179 (BioLegend Cat. No. 134005) AB_1626175 (BioLegend Cat. No. 134006)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
- Distribution
-
Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.
- Function
- Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
- Ligand/Receptor
- Galectin -9
- Cell Type
- Dendritic cells, Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454
3. Mooney L, et al. 2002. Nature. 415:536
4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259
- Gene ID
- 171285 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
