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Purified anti-human CD16 Antibody
产品名称：
Purified anti-human CD16 Antibody
产品类别：
抗体
产品编号：
360701
产品应用：
360701
[价格]
规格 价格 库存
25µg ￥ 780 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK cell-enriched fraction from human peripheral blood.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G84.

Donor variability has been observed for clone B73.1 staining1, especially on granulocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Perussia B, et al. 1983. J. Immunol. 130:2133.
  2. Lanier LL, et al. 1985. J. Exp. Med. 162:2089.
  3. Perussia B, et al. 1984. J. Immunol. 133:180.
  4. Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)
Product Citations
  1. Sigal N, et al. 2022. Methods Mol Biol. 2543:113. PubMed
  2. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  3. Cheung P, et al. 2018. Cell. 173:1385. PubMed
  4. Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. PubMed
  5. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  6. Waugh KA, et al. 2020. Cell Reports. 29(7):1893-1908.e4.. PubMed
RRID
AB_2562692 (BioLegend Cat. No. 360701) AB_2562693 (BioLegend Cat. No. 360702)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
IgG Fc receptor III (FcγRIII)
Cell Type
Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
5. Bj?rkstr?m NK, et al. 2008. J. Immonol. 181:4219.

Gene ID
2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

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