PE/Cyanine7 anti-human CD16 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1908	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NK cell-enriched fraction from human peripheral blood.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G8⁴.

Donor variability has been observed for clone B73.1 staining¹, especially on granulocytes.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Perussia B, et al. 1983. J. Immunol. 130:2133.
Lanier LL, et al. 1985. J. Exp. Med. 162:2089.
Perussia B, et al. 1984. J. Immunol. 133:180.
Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)

Product Citations

Rasmussen EB, et al. 2021. Clin Exp Gastroenterol. 14:259. PubMed
Bsat M, et al. 2021. Curr Protoc. 1:e74. PubMed
Rice TF, et al. 2021. EBioMedicine. 72:103612. PubMed
Liang Y, et al. 2019. J Allergy Clin Immunol. 144:1025. PubMed
Sharma N,et al. 2017. J Immunol. 10.4049/jimmunol.1700647. PubMed
Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
Rotolo A et al. 2018. Cancer cell. 34(4):596-610 . PubMed
Yang C, et al. 2019. Nat Commun. 10:3931. PubMed

RRID

AB_2562950 (BioLegend Cat. No. 360707) AB_2562951 (BioLegend Cat. No. 360708)

Antigen Details

Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: IgG Fc receptor III (FcγRIII)
Cell Type: Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
5. Bj?rkstr?m NK, et al. 2008. J. Immonol. 181:4219.
Gene ID: 2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt: View information about CD16 on UniProt.org

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