Brilliant Violet 605™ anti-human CD16 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2580	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NK cell-enriched fraction from human peripheral blood.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G8⁴.

Donor variability has been observed for clone B73.1 staining¹, especially on granulocytes.

Application References (PubMed link indicates BioLegend citation)

Perussia B, et al. 1983. J. Immunol. 130:2133.
Lanier LL, et al. 1985. J. Exp. Med. 162:2089.
Perussia B, et al. 1984. J. Immunol. 133:180.
Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)

RRID

AB_2650663 (BioLegend Cat. No. 360727) AB_2650664 (BioLegend Cat. No. 360728)

Antigen Details

Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: IgG Fc receptor III (FcγRIII)
Cell Type: Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
5. Bj?rkstr?m NK, et al. 2008. J. Immonol. 181:4219.
Gene ID: 2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt: View information about CD16 on UniProt.org

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