Alexa Fluor® 700 anti-human CD16 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1764	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NK cell-enriched fraction from human peripheral blood.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G8⁴.

Donor variability has been observed for clone B73.1 staining¹, especially on granulocytes.

Application References (PubMed link indicates BioLegend citation)

Perussia B, et al. 1983. J. Immunol. 130:2133.
Lanier LL, et al. 1985. J. Exp. Med. 162:2089.
Perussia B, et al. 1984. J. Immunol. 133:180.
Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)

RRID

AB_2563072 (BioLegend Cat. No. 360717) AB_2563073 (BioLegend Cat. No. 360718)

Antigen Details

Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: IgG Fc receptor III (FcγRIII)
Cell Type: Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
5. Bj?rkstr?m NK, et al. 2008. J. Immonol. 181:4219.
Gene ID: 2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt: View information about CD16 on UniProt.org

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