Purified anti-mouse CD23 (Maxpar® Ready) Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1416	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding^2-4, immunohistochemical staining of acetone-fixed frozen sections⁵, and spatial biology (IBEX)^7,8.

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2563731 (BioLegend Cat. No. 101625)

Antigen Details

Structure: C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution: B cells, follicular dendritic cells
Function: Regulates B cell activation
Ligand/Receptor: IgE, CD19/CD21/CD81
Cell Type: B cells, Dendritic cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
Gene ID: 14128 View all products for this Gene ID
UniProt: View information about CD23 on UniProt.org

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