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PE anti-mouse CD23 Antibody
产品名称：
PE anti-mouse CD23 Antibody
产品类别：
抗体
产品编号：
101607
产品应用：
101607
[价格]
规格 价格 库存
50µg ￥ 1320 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
  2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
  3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
  4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
  5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
  6. Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
  7. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  8. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Chiu H, et al. 2021. iScience. 24:102748. PubMed
  2. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  3. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  4. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  5. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  6. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  7. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  8. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  9. Lee-Chang C, et al. 2016. J Immunol. 196: 3385-3397. PubMed
  10. McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed
  11. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  12. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  13. Zhao X, et al. 2017. Nat Med. 10.1038/nm.4260. PubMed
  14. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  15. Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed
  16. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  17. Wang D, et al. 2005. Int Immunol. 1.527083333. PubMed
  18. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  19. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  20. Smith LK, et al. 2021. Elife. 10:. PubMed
  21. Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed
  22. Haines RR, et al. 2019. J Immunol. 203:1867. PubMed
  23. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  24. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID
AB_312832 (BioLegend Cat. No. 101607) AB_312833 (BioLegend Cat. No. 101608)

Antigen Details

Structure
C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution

B cells, follicular dendritic cells

Function
Regulates B cell activation
Ligand/Receptor
IgE, CD19/CD21/CD81
Cell Type
B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.

Gene ID
14128 View all products for this Gene ID
UniProt
View information about CD23 on UniProt.org

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