Pacific Blue™ anti-mouse CD23 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1344	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application Notes

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding^2-4, immunohistochemical staining of acetone-fixed frozen sections⁵, and spatial biology (IBEX)^7,8.

Application References (PubMed link indicates BioLegend citation)

Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Thomsen I, et al. 2021. J Immunol. Online ahead of print. PubMed
Joly M, et al. 2014. J Immunol. 193:3947. PubMed
Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
Prokopec K, et al. 2016. J Immunol. 197: 2063 - 2068. PubMed
Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed
Noviski M, et al. 2018. Elife. 7:e35074. PubMed
Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
Roberto MP, et al. 2021. Immunity. 54(8):1807-1824.e14. PubMed
Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed

RRID

AB_2103307 (BioLegend Cat. No. 101615) AB_2103306 (BioLegend Cat. No. 101616)

Antigen Details

Structure: C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution: B cells, follicular dendritic cells
Function: Regulates B cell activation
Ligand/Receptor: IgE, CD19/CD21/CD81
Cell Type: B cells, Dendritic cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
Gene ID: 14128 View all products for this Gene ID
UniProt: View information about CD23 on UniProt.org

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