Alexa Fluor® 700 anti-mouse CD23 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1272	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding^2-4, immunohistochemical staining of acetone-fixed frozen sections⁵, and spatial biology (IBEX)^7,8.

Application References (PubMed link indicates BioLegend citation)

Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed

RRID

AB_2687124 (BioLegend Cat. No. 101631) AB_2687125 (BioLegend Cat. No. 101632)

Antigen Details

Structure: C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution: B cells, follicular dendritic cells
Function: Regulates B cell activation
Ligand/Receptor: IgE, CD19/CD21/CD81
Cell Type: B cells, Dendritic cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
Gene ID: 14128 View all products for this Gene ID
UniProt: View information about CD23 on UniProt.org

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