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Purified anti-GFP Epitope Tag Antibody (Previously
产品名称:
Purified anti-GFP Epitope Tag Antibody (Previously
产品类别:
抗体
产品编号:
902605
产品应用:
902605
[价格]
规格 价格 库存
25µL ¥ 1068 1

产品详情

Product Details

Verified Reactivity
Green Fluorescent Protein Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Antibody B34, generated against purified wild type GFP, offers an alternitive for detecting GFP and GFP variants including enhanced/mutant GFP.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, Purification

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
? WB: 1:5,000*
?

Application Notes

This antibody is effective in immunoblotting (WB) of GFP labeled proteins.

*Predicted MW = 27 kD

Application References

(PubMed link indicates BioLegend citation)
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  2. Kessels MM, et al. 2001. J Cell Biol. 153:351.
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  5. Chalfie M. 1995. Photochem Photobio. 62:651.
  6. Stearns T. 1995. Curr Biol. 5:262.
  7. Chalfie M, et al. 1994. Science. 263:802.
  8. Lavy M, et al. 2007. Curr. Biol. 17:947. (WB)
  9. Hobl B, et al. 2013. Protein Expr. Purif. 10:32. (WB)
  10. Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed
  11. Asante D, et al. 2013. J. Cell. Sci. 126:5189. (WB) PubMed
  12. Oh Y, et al. 2013. Mol Biol Cell. 24:1305. (WB) PubMed
  13. Wloka C, et al. 2013. J. Cell. Biol. 200:271. (WB)
  14. Redfern RE, et al. 2010. Protein Sci. 19:1948. (WB) PubMed
Product Citations
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  13. Stehle D, et al. 2018. Sci Rep. 8:12434. PubMed
  14. Bhatter N, et al. 2019. FEBS J. 286:4693. PubMed
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  17. Kennedy SP, et al. 2019. Breast Cancer Res. 21:43. PubMed
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  23. Chattopadhyay S, et al. 2022. Nat Commun. 13:7184. PubMed
  24. Wang Y, et al. 2016. Sci Rep. 6:37213. PubMed
  25. Zhang Y, Barbieri Q 2007. J Biol Chem. 282:13022-13032. PubMed
  26. Schmitz F, et al. 2021. Sci Rep. 11:19232. PubMed
  27. Basic M, et al. 2021. J Biol Chem. 297:101263. PubMed
  28. Liu Y, et al. 2020. Nat Commun. 11:589. PubMed
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  32. Robles‐Valero J et al. 2017. Cancer cell. 32(5):608-623 . PubMed
  33. Zhang X, et al. 2016. Nat Commun. 7:12109. PubMed
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  35. den Brave F, et al. 2020. Cell Rep. 31:107680. PubMed
  36. McCaughey J, et al. 2019. J Cell Biol. 218:929. PubMed
RRID
AB_2734671 (BioLegend Cat. No. 902605) AB_2565021 (BioLegend Cat. No. 902601) AB_2565022 (BioLegend Cat. No. 902602)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about GFP on UniProt.org

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