Brilliant Violet 421™ anti-human IFN-γ-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2376	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Baboon, Cynomolgus, Rhesus, African Green, Pigtailed Macaque, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

E. coli-expressed recombinant human IFN-γ

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Flow Cytometry²: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-? -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization^1,3,6,7: The Ultra-LEAF? Purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 506531).

Application References (PubMed link indicates BioLegend citation)

Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
Kaur A, et al. 2002. J Virol. 76:3646.
Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)

Product Citations

Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
Yuan Y, et al. 2019. Cancer Med. 8:3811. PubMed

RRID

AB_2801097 (BioLegend Cat. No. 506537) AB_2801098 (BioLegend Cat. No. 506538)

Antigen Details

Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8⁺ and CD4⁺ T cells, NK cells
Cell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID: 3458 View all products for this Gene ID
UniProt: View information about IFN-gamma on UniProt.org

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