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Alexa Fluor® 700 anti-human IFN-γ Antibo
产品名称：
Alexa Fluor® 700 anti-human IFN-γ Antibo
产品类别：
抗体
产品编号：
506515
产品应用：
506515
[价格]
规格 价格 库存
25µg ￥ 1512 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, African Green, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed recombinant human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Flow Cytometry2: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-? -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,3,6,7: The Ultra-LEAF? Purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 506531).

Application References

(PubMed link indicates BioLegend citation)
  1. Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
  2. Kaur A, et al. 2002. J Virol. 76:3646.
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  5. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  6. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
  7. Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)
Product Citations
  1. Gagne M, et al. 2022. Cell. 185:113. PubMed
  2. Mandolesi M, et al. 2021. Cell Reports Medicine. 2(4):100252. PubMed
  3. D, et al. 2016. Tuberculosis. 95: 470-475. PubMed
  4. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  5. Nishimura Y, et al. 2017. Nature. 543:559. PubMed
  6. Pattekar A, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 11(5):1267-1289. PubMed
  7. Tchakoute C, et al. 2015. J Infect Dis. 211:338. PubMed
  8. Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
  9. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  10. Cordonnier M, et al. 2020. J Extracell Vesicles. 9:1710899. PubMed
  11. King HAD, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  12. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  13. Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
  14. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_961353 (BioLegend Cat. No. 506515) AB_961351 (BioLegend Cat. No. 506516)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

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