Brilliant Violet 421™ anti-mouse TCR V&alpha
- 产品名称：
- Brilliant Violet 421™ anti-mouse TCR V&alpha
- 产品类别：
- 抗体
- 产品编号：
- 127825
- 产品应用：
- 127825
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Soluble TCR from mouse CTL clone KB5-C20
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
- Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
- Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
- Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
- Rao RR, et al. 2012. Immunity. 36:374. PubMed.
- Product Citations
-
- Pasciuto E, et al. 2020. Cell. 182:625. PubMed
- RRID
- AB_2814020 (BioLegend Cat. No. 127825)
- Distribution
-
On a subset of T cells
- Function
- In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
- Interaction
- Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
- Ligand/Receptor
- TCR recognizes MHC/peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Kubo RT, et al. 1989. J. Immunol.. 142:2736.
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.
- Gene ID
- 21473 View all products for this Gene ID
- UniProt
- View information about TCR Valpha2 on UniProt.org
