Alexa Fluor® 700 anti-mouse TCR Vα2 Anti
- 产品名称：
- Alexa Fluor® 700 anti-mouse TCR Vα2 Anti
- 产品类别：
- 抗体
- 产品编号：
- 127823
- 产品应用：
- 127823
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Soluble TCR from mouse CTL clone KB5-C20
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
- Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
- Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
- Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
- Rao RR, et al. 2012. Immunity. 36:374. PubMed.
- Product Citations
-
- Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
- RRID
- AB_2814018 (BioLegend Cat. No. 127823) AB_2814019 (BioLegend Cat. No. 127824)
- Distribution
-
On a subset of T cells
- Function
- In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
- Interaction
- Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
- Ligand/Receptor
- TCR recognizes MHC/peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Kubo RT, et al. 1989. J. Immunol.. 142:2736.
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.
- Gene ID
- 21473 View all products for this Gene ID
- UniProt
- View information about TCR Valpha2 on UniProt.org
