Alexa Fluor® 647 anti-mouse TCR Vα2 Anti
- 产品名称：
- Alexa Fluor® 647 anti-mouse TCR Vα2 Anti
- 产品类别：
- 抗体
- 产品编号：
- 127812
- 产品应用：
- 127812
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Soluble TCR from mouse CTL clone KB5-C20
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
- Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
- Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
- Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
- Rao RR, et al. 2012. Immunity. 36:374. PubMed.
- Product Citations
-
- Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
- Allen G 2009. J Immunol. 182:6639. PubMed
- Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
- RRID
- AB_1186115 (BioLegend Cat. No. 127812)
- Distribution
-
On a subset of T cells
- Function
- In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
- Interaction
- Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
- Ligand/Receptor
- TCR recognizes MHC/peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Kubo RT, et al. 1989. J. Immunol.. 142:2736.
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.
- Gene ID
- 21473 View all products for this Gene ID
- UniProt
- View information about TCR Valpha2 on UniProt.org
