Ultra-LEAF™ Purified anti-mouse / rat IL-1&b-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli -expressed, recombinant mouse IL-1β

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture³: The purified B122 antibody is useful as the capture antibody in a mouse IL-1? sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture.

Certain formats of clone B122 have been reported for in vitro neutralization of IL-1? bioactivity¹, in vivo cytokine depletion⁵, immunohistochemical staining of frozen sections⁶, Western blotting⁷, and the study of endotoxin shock⁸.

Application References (PubMed link indicates BioLegend citation)

Hogquist K, et al. 1991. J. Immunol. 146:1534.
Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed

Product Citations

Burke TP, et al. 2020. Nat Microbiol. 0.686111111. PubMed
Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
Viladomiu M, et al. 2021. Cell Host Microbe. 29(4):607-619.e8. PubMed
Dani N, et al. 2021. Cell. 184(11):3056-3074.e21. PubMed

RRID

AB_2814399 (BioLegend Cat. No. 503513) AB_2814400 (BioLegend Cat. No. 503514) AB_2814401 (BioLegend Cat. No. 503515) AB_2814402 (BioLegend Cat. No. 503516) AB_2814403 (BioLegend Cat. No. 503517) AB_2814404 (BioLegend Cat. No. 503518)

Antigen Details

Structure: Cytokine; 17.5 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin
Gene ID: 16176 View all products for this Gene ID
UniProt: View information about IL-1beta on UniProt.org

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