Biotin anti-mouse / rat IL-1β Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1344	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli -expressed, recombinant mouse IL-1β

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.

Application Notes

ELISA or ELISPOT Capture³: The purified B122 antibody is useful as the capture antibody in a mouse IL-1? sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture.

Certain formats of clone B122 have been reported for in vitro neutralization of IL-1? bioactivity¹, in vivo cytokine depletion⁵, immunohistochemical staining of frozen sections⁶, Western blotting⁷, and the study of endotoxin shock⁸.

Application References (PubMed link indicates BioLegend citation)

Hogquist K, et al. 1991. J. Immunol. 146:1534.
Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed

Product Citations

Bulek K, et al. 2020. J Clin Invest. 130:4218. PubMed
Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed

RRID

AB_2295906 (BioLegend Cat. No. 503505)

Antigen Details

Structure: Cytokine; 17.5 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin
Gene ID: 16176 View all products for this Gene ID
UniProt: View information about IL-1beta on UniProt.org

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