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PE anti-human TCR γ/δ Antibody
产品名称：
PE anti-human TCR γ/δ Antibody
产品类别：
抗体
产品编号：
331210
产品应用：
331210
[价格]
规格 价格 库存
100tests ￥ 3216 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Pigtailed Macaque
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone B1 is also known as clone B1.1.

Additional reported applications (for the relevant formats) include:?immunohistochemical staining of acetone-fixed frozen sections3 and paraffin-embedded sections5, in vitro blocking, and spatial biology (IBEX)8,9. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for highly sensitive assays (Cat. Nos. 331235 and 331236).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Rodriguez-Gago M, et al. 2001. Transplantation. 72:503.
  2. Lehmann FS, et al. 2002. Am. J. Physiol. Gastrointest. Liver. Physiol. 283:G481. (FC)
  3. Bordignon M, et al. 2008. Mol. Med. Rep. 1:485. (IHC)
  4. Conrad M, et al. 2007. Cytom. Part A 71A:925. (FC)
  5. Pollinger B, et al. 2011. J. Immunol. 186:2602. (IHC)
  6. Correia DV, et al. 2011. Blood. 118:992. (Block)
  7. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  8. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  9. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed
  2. Laurent A, et al. 2014. PLoS One. 9:103683. PubMed
  3. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  4. Trabanelli S, et al. 2017. Nat Commun. 10.1038/s41467-017-00678-2. PubMed
  5. Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
  6. Chen Z, et al. 2021. Dis Markers. 2021:5838582. PubMed
  7. Du B, et al. 2020. Scand J Immunol. 91:e12872. PubMed
  8. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
RRID
AB_1089219 (BioLegend Cat. No. 331209) AB_1089218 (BioLegend Cat. No. 331210)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex
Distribution

T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum

Function
Antigen recognition
Ligand/Receptor
Some bacterial or tumor antigens bound MHC class I, CD1 molecule
Cell Type
Epithelial cells, T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Lanier LL, et al. 1987. J. Clin. Immunol. 7:429.
2. Spencer J, et al. 1989. Eur. J. Immunol. 19:1335.
3. Uyemura K, et al. 1991. J. Exp. Med. 174:683.
4. Spada FM, et al. 2000. J. Exp. Med. 191:907.

Gene ID
6964 View all products for this Gene ID 6965 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org

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