Brilliant Violet 510™ anti-human TCR γ-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2448	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Pigtailed Macaque

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone B1 is also known as clone B1.1.

Additional reported applications (for the relevant formats) include:?immunohistochemical staining of acetone-fixed frozen sections³ and paraffin-embedded sections⁵, in vitro blocking, and spatial biology (IBEX)^8,9. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for highly sensitive assays (Cat. Nos. 331235 and 331236).

Application References (PubMed link indicates BioLegend citation)

Rodriguez-Gago M, et al. 2001. Transplantation. 72:503.
Lehmann FS, et al. 2002. Am. J. Physiol. Gastrointest. Liver. Physiol. 283:G481. (FC)
Bordignon M, et al. 2008. Mol. Med. Rep. 1:485. (IHC)
Conrad M, et al. 2007. Cytom. Part A 71A:925. (FC)
Pollinger B, et al. 2011. J. Immunol. 186:2602. (IHC)
Correia DV, et al. 2011. Blood. 118:992. (Block)
Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Arunachalam PS, et al. 2021. Nature. 596:410. PubMed
Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed
Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
Ireland RE, et al. 2022. Viruses. 14:. PubMed
Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed

RRID

AB_2562637 (BioLegend Cat. No. 331219) AB_2564275 (BioLegend Cat. No. 331220)

Antigen Details

Structure: Ig superfamily, associates with CD3 complex
Distribution: T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function: Antigen recognition
Ligand/Receptor: Some bacterial or tumor antigens bound MHC class I, CD1 molecule
Cell Type: Epithelial cells, T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Lanier LL, et al. 1987. J. Clin. Immunol. 7:429.
2. Spencer J, et al. 1989. Eur. J. Immunol. 19:1335.
3. Uyemura K, et al. 1991. J. Exp. Med. 174:683.
4. Spada FM, et al. 2000. J. Exp. Med. 191:907.
Gene ID: 6964 View all products for this Gene ID 6965 View all products for this Gene ID
UniProt: View information about TCR gamma/delta on UniProt.org

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