TotalSeq™-A0132 anti-human EGFR Antibody -上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Non-small cell lung cancer (NSCLC) cell line NCI-H322

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-A oligomer under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CCTTGGCACCCGAGAATTCCA (PCR handle), and BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A (capture sequence). B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our?Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92.

Product Citations

Sun L, et al. 2021. Sci Rep. 11:12477. PubMed

RRID

AB_2734373 (BioLegend Cat. No. 352923)

Antigen Details

Structure: Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
Distribution: Epithelial cells and endothelial cells; frequently found in epithelial carcinomas
Function: Controls cell growth and differentiation
Interaction: MAPK, Akt, JNK
Ligand/Receptor: Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
Cell Type: Endothelial cells, Epithelial cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: CD Molecules, Growth Factors
Antigen References: 1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49.
2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522.
3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415.
4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760.
Gene ID: 1956 View all products for this Gene ID
UniProt: View information about EGFR on UniProt.org

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