PE anti-mouse CD309 (VEGFR2, Flk-1) Antibody
- 产品名称：
- PE anti-mouse CD309 (VEGFR2, Flk-1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 136403
- 产品应用：
- 136403
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine Flk1 fused to hIgG Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Avas12 recognizes a different epitope than clone 89B3A5. Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining of paraformaldehyde-fixed frozen sections2.
- Application References
(PubMed link indicates BioLegend citation) -
- Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. (WB)
- Ishitobi H, et al. 2010. Exp. Anim. 59:615. (IHC-F)
- Product Citations
-
- Gong H, et al. 2016. Stem Cell Reports. 7:1050-1058. PubMed
- Kinoshita M, et al. 2020. Cell Stem Cell. . PubMed
- RRID
- AB_1967093 (BioLegend Cat. No. 136403) AB_1967124 (BioLegend Cat. No. 136404)
- Structure
- A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily
- Distribution
-
Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle
- Function
- Plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability
- Ligand/Receptor
- Vascular endothelial growth factor (VEGF)
- Biology Area
- Angiogenesis, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Kaburn N, et al. 1997. Development. 124:2039
2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111
3. Nishikawa SI, et al. 1998. Immunity 8 (6):761
4. Shalaby F, et al. 1997. Cell. 89 (6):981
- Gene ID
- 16542 View all products for this Gene ID
- UniProt
- View information about CD309 on UniProt.org
