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Purified anti-AU5 Epitope Tag Antibody (Previously
产品名称：
Purified anti-AU5 Epitope Tag Antibody (Previously
产品类别：
抗体
产品编号：
902007
产品应用：
902007
[价格]
规格 价格 库存
25µL ￥ 1068 1

产品详情

Product Details

Verified Reactivity
TDFYLK Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The AU5 monoclonal antibody was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope TDFYLK.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, ICC, IP, Purification
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
? WB: 1:500*
? ICC: 1:500
? IP: 1:150

Application Notes

This antibody is effective in immunofluorescence (IF), immunoblotting, and immunoprecipitation (IP) of tagged proteins.

This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.

Application References

(PubMed link indicates BioLegend citation)
  1. Burke TW, et al. 2001. J Biol Chem. 276:15397.
  2. Marinissen MJ, et al. 2001. Genes Dev. 15:535.
  3. Lim PS, et al. 1990. J Infect Dis. 162:1263.
Product Citations
  1. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
RRID
AB_2734668 (BioLegend Cat. No. 902007) AB_2565015 (BioLegend Cat. No. 902002)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about AU5 on UniProt.org

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