Purified anti-AU1 Epitope Tag Antibody (Previously
- 产品名称：
- Purified anti-AU1 Epitope Tag Antibody (Previously
- 产品类别：
- 抗体
- 产品编号：
- 901905
- 产品应用：
- 901905
- Verified Reactivity
- DTYRYI Tag
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Monoclonal antibody AU1 was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope DTYRYI.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB, ICC, IP, Purification
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
? WB: 1:1,000
? ICC: 1:1,000
? IP: 1:150
- Application Notes
This antibody is effective in immunofluorescence (IF), immunoblotting (WB), and immunoprecipitation (IP) of tagged proteins.
The clone was specifically designed for use in epitope tagging. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.
- Application References
(PubMed link indicates BioLegend citation) -
- Mothe-Satney I, et al. 2000. J Biol Chem. 275:33836.
- Goldstein DJ, et al. 1992. EMBO J. 11:4851.
- Lim PS, et al. 1990. J Infect Dis. 162:1263.
- Sauter D, et al. 2011. PLoS Pathog. 8:e1003093. (WB, IF)
- Kluge SF, et al. 2013. Retrovirology 10:32. (WB, IF) PubMed
- Heigele A, et al. 2012. J. Virol. 86:36. WB) PubMed
- Product Citations
-
- Heigele A, et al. 2012. J Virol. 86:36-48. PubMed
- Waise TMZ, et al. 2019. Nat Commun. 10:714. PubMed
- Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
- Kluge S, et al. 2013. Retrovirology. 10:32. PubMed
- Kim YJ, et al. 2021. PLoS Pathog. 17:e1009179. PubMed
- Chen X, et al. 2021. Sci Rep. 11:14525. PubMed
- Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
- RRID
- AB_2734667 (BioLegend Cat. No. 901905) AB_2565013 (BioLegend Cat. No. 901901) AB_2565014 (BioLegend Cat. No. 901902)
