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Purified anti-rat CD252 (OX-40 Ligand) Antibody
产品名称:
Purified anti-rat CD252 (OX-40 Ligand) Antibody
产品类别:
抗体
产品编号:
200501
产品应用:
200501
[价格]
规格 价格 库存
50µg ¥ 1128 1

产品详情

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ACI-S1 rat T-cell lymphoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking the binding of OX-40 to OX-40L and the inhibition of T cell costimulatory activity of OX40L1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 200506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 200504) or biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200508).

Application References

(PubMed link indicates BioLegend citation)
  1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
RRID
AB_2571881 (BioLegend Cat. No. 200501)

Antigen Details

Structure
TNF superfamily, 22 kD
Distribution
Dendritic cells, activated B cells
Function
T cell dependent humoral responses
Ligand/Receptor
OX40 (CD134)
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
2. Akiba, H., et al. 1998. Biochem. Biophys. Res. Commun. 251:131.
3. Akiba, H., et al. 2000. J. Exp. Med. 191:375.

Gene ID
22164 View all products for this Gene ID
UniProt
View information about CD252 on UniProt.org

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