Purified anti-human IRF3 Antibody
- 产品名称：
- Purified anti-human IRF3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 395902
- 产品应用：
- 395902
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human IRF-3 protein (entire protein)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Rustagi A, et al. 2012. Methods. 59(2):225-32
- RRID
- AB_2810597 (BioLegend Cat. No. 395902)
- Structure
- Human IRF-3 has 427 amino acids with predicted Mw 47.2 kD and observed Mw 52 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9 kD and observed Mw 45 kD.
- Distribution
-
Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.
- Function
- CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Pattwell SS, et al. 2017. Trends Mol Med. (9):773-776
- Zhang ZX, et al. 2017. Leuk Lymphoma. 58(3): 646-654
- Zhao B, et al. 2016. Proc Natl Acad Sci USA. 113(24)E3403-12
- Gene ID
- 3661 View all products for this Gene ID
- UniProt
- View information about IRF3 on UniProt.org
