Ultra-LEAF™ Purified anti-mouse CD140b Antib
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD140b Antib
- 产品类别：
- 抗体
- 产品编号：
- 136011
- 产品应用：
- 136011
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PDGFR-β-hIgG1 recombinant fusion protein
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported (for the relevant formats) applications include: Western blotting and blocking function2. The Ultra-LEAF? purified antibody is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Sano H, et al. 2001. Circulation 103:2955.
- Sano H, et al. 2002. Am. J. Pathol. 161:135. (Block)
- RRID
- AB_2832491 (BioLegend Cat. No. 136011) AB_2832492 (BioLegend Cat. No. 136012)
- Structure
- Beta chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
- Distribution
-
Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
- Function
- Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
- Ligand/Receptor
- PDGFs
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Soriano P, et al. 1994. Genes Dev. 8:1888
2. Takakura N, et al. 1996. J Invest Dermatol. 107:770
3. Yarden Y, et al. 1986. Nature 323:226
- Gene ID
- 18596 View all products for this Gene ID
- UniProt
- View information about CD140b on UniProt.org
