PE anti-mouse CD140a Antibody
- 产品名称：
- PE anti-mouse CD140a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 135905
- 产品应用：
- 135905
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PDGFR-α-hIgG1 recombinant fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF? purified antibody is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
- Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
- Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
- Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
- Product Citations
-
- Buechler MB, et al. 2021. Nature. 593:575. PubMed
- Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
- Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
- Zelic M, et al. 2021. Cell Reports. 35(6):109112. PubMed
- Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
- Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed
- Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
- Salzer MC et al. 2018. Cell. 175(6):1575-1590 . PubMed
- Chen M, et al. 2020. Sci Adv. 6:eaax9605. PubMed
- Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
- Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
- Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed
- Lu A, et al. 2020. Sci Rep. 10:7075. PubMed
- Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
- Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
- Frondelli MJ, et al. 2021. Neurotrauma Rep. 2:285. PubMed
- Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
- Diekmann U, et al. 2019. Sci Rep. 9:996. PubMed
- Ito K, et al. 2014. J Biol Chem. 289:29892. PubMed
- Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
- Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed
- Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
- Frech S, et al. 2021. J Invest Dermatol. . PubMed
- Xi W, et al. 2021. Stem Cell Res Ther. 167:12. PubMed
- Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
- Fung CW, et al. 2022. Cell Discov. 8:61. PubMed
- Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
- Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed
- Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
- Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
- Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed
- Shibamoto M, et al. 2019. Int Heart J. 60:944. PubMed
- Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
- Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
- Konishi S, et al. 2022. STAR Protoc. 3:101447. PubMed
- Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
- Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
- Maller O, et al. 2020. Nat Mater. 20:548. PubMed
- Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
- Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
- Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
- Kwon H, Kim S 2016. Cell Death Dis. 7:22285. PubMed
- Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
- Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
- Wang Y, et al. 2020. Cell Reports. 31(5):107600. PubMed
- Velloso FJ, et al. 2022. STAR Protoc. 3:101065. PubMed
- Velletri T, et al. 2020. Cell Death Differ. . PubMed
- Bravo JI, et al. 2021. STAR Protocols. 2(2):100406. PubMed
- Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
- Ferrari N, et al. 2019. Nat Commun. 10:130. PubMed
- RRID
- AB_1953268 (BioLegend Cat. No. 135905) AB_1953269 (BioLegend Cat. No. 135906)
- Structure
- Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
- Distribution
-
Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
- Function
- Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
- Ligand/Receptor
- PDGFs
- Cell Type
- Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883
- Gene ID
- 18595 View all products for this Gene ID
- UniProt
- View information about CD140a on UniProt.org
