Ultra-LEAF™ Purified anti-mouse IFN-γ -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2376	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse IFN-γ protein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.25 - 2.0 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 3.9 to 500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detecting antibody, and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture.

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References (PubMed link indicates BioLegend citation)

Prat M, et al. 1984. P. Natl. Acad. Sci. USA 81:4515. (IP)
Kort JJ, et al. 2006. J. Neuroimmunol. 173:23. (ELISPOT) PubMed
Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed

Product Citations

Jia X, et al. 2021. Front Cell Dev Biol. 9:685522. PubMed

RRID

AB_2810651 (BioLegend Cat. No. 517905) AB_2810652 (BioLegend Cat. No. 517906)

Antigen Details

Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
Gene ID: 15978 View all products for this Gene ID
UniProt: View information about IFN-gamma on UniProt.org

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