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Purified anti-mouse IFN-γ Antibody
产品名称:
Purified anti-mouse IFN-γ Antibody
产品类别:
抗体
产品编号:
517901
产品应用:
517901
[价格]
规格 价格 库存
50µg ¥ 1068 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IFN-γ protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
ELISPOT, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5?- 4.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein (Cat. No. 575309) are recomended at concentration up to 2000 pg/mL. The final standard range should be determined by the end-user.. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detection?antibody, and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture.

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Prat M, et al. 1984. P. Natl. Acad. Sci. USA 81:4515. (IP)
  2. Kort JJ, et al. 2006. J. Neuroimmunol. 173:23. (ELISPOT) PubMed
  3. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  2. Adhikari AS, et al. 2022. Front Oncol. 12:850546. PubMed
  3. Ranjan K, et al. 2021. J Clin Invest. 131:. PubMed
  4. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
RRID
AB_10900246 (BioLegend Cat. No. 517901) AB_10900809 (BioLegend Cat. No. 517902)

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

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