PE anti-human CD129 (IL-9 R) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 3648	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: inhibition the binding of IL-9 to the high affinity a-chain of the human IL-9 receptor^1,2, and ELISA for detection of soluble IL-9R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 310404) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 310408).

Application References (PubMed link indicates BioLegend citation)

De Smedt M, et al. 2000. J. Immunol. 164:1761.
Pilette C, et al. 2002. J. Immunol. 168:4103.
Demoulin JB, et al.1996. Mol. Cell. Biol. 16 (9):4710.

Product Citations

Das S, et al. 2021. J Immunol. 206:2740. PubMed
Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
Xiao M, et al. 2017. Blood. 129:3196. PubMed

RRID

AB_314816 (BioLegend Cat. No. 310403) AB_314817 (BioLegend Cat. No. 310404)

Antigen Details

Structure: Cytokine receptor family, type I membrane protein or soluble, associate with common γ chain(CD132), 57 kD
Distribution: Eosinophils, mast, thymocytes, erythroid, myeloid progenitors, macrophages
Function: Activates STAT; critical for intrathymic T cell development
Ligand/Receptor: IL-9
Cell Type: Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Demoulin JB, et al.1996. Mol. Cell. Biol. 16:4710.
Gene ID: 3581 View all products for this Gene ID
UniProt: View information about CD129 on UniProt.org

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