Ultra-LEAF™ Purified anti-mouse CD115 (CSF-1
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD115 (CSF-1
- 产品类别：
- 抗体
- 产品编号：
- 135537
- 产品应用：
- 135537
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.?Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.
- Application References
(PubMed link indicates BioLegend citation) -
- Sudo T, et al. 1995. Oncogene 11:2469.
- Murayama T, et al. 1999. Circulation 99:1740.
- Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
- Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
- Dong L, et al. 2016. Nature. 539:304–308. PubMed
- RRID
- AB_2832481 (BioLegend Cat. No. 135537) AB_2832482 (BioLegend Cat. No. 135538) AB_2832483 (BioLegend Cat. No. 135539) AB_2832484 (BioLegend Cat. No. 135540) AB_2832485 (BioLegend Cat. No. 135541) AB_2832486 (BioLegend Cat. No. 135542)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Macrophage colony stimulating factor (M-CSF), IL-34
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Sudo T, et al. 1995 Oncogene 11:2469.
- Murayama T, et al. 1999 Circulation 99:1740.
- Goswami S, et al. 2005 Cancer Res. 65:5278.
- Yu W, et al. 2008 J. Leuko. Biol. 84:852.
- Gene ID
- 12978 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org
