Purified anti-mouse CD115 (CSF-1R) Antibody
- 产品名称：
- Purified anti-mouse CD115 (CSF-1R) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 135501
- 产品应用：
- 135501
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.?Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.
- Application References
(PubMed link indicates BioLegend citation) -
- Sudo T, et al. 1995. Oncogene 11:2469.
- Murayama T, et al. 1999. Circulation 99:1740.
- Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
- Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
- Dong L, et al. 2016. Nature. 539:304–308. PubMed
- Product Citations
-
- Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
- Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Kapellos TS, et al. 2017. Mediators Inflamm. 2017:4315412. PubMed
- Inclan-Rico JM, et al. 2020. PLoS Pathog. 16:e1008579. PubMed
- Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
- Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed
- Jaeger B, et al. 2012. J Exp Med. 209:565. PubMed
- Winter C, et al. 2018. Cell Metab. 28:175. PubMed
- Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
- Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
- Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
- Seehus CR, et al. 2017. Nat Commun. 8:1900. PubMed
- RRID
- AB_1937292 (BioLegend Cat. No. 135501) AB_1937293 (BioLegend Cat. No. 135502)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Macrophage colony stimulating factor (M-CSF), IL-34
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Sudo T, et al. 1995 Oncogene 11:2469.
- Murayama T, et al. 1999 Circulation 99:1740.
- Goswami S, et al. 2005 Cancer Res. 65:5278.
- Yu W, et al. 2008 J. Leuko. Biol. 84:852.
- Gene ID
- 12978 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org
