Biotin anti-mouse CD115 (CSF-1R) Antibody
- 产品名称：
- Biotin anti-mouse CD115 (CSF-1R) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 135508
- 产品应用：
- 135508
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.?Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.
- Application References
(PubMed link indicates BioLegend citation) -
- Sudo T, et al. 1995. Oncogene 11:2469.
- Murayama T, et al. 1999. Circulation 99:1740.
- Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
- Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
- Dong L, et al. 2016. Nature. 539:304–308. PubMed
- Product Citations
-
- Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
- H?uselmann I, et al. 2016. Cancer Res. 76: 5302 - 5312. PubMed
- Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed
- Hoos A, et al. 2014. Cancer Res. 74:695. PubMed
- Winkler MJ, et al. 2020. Basic Res Cardiol. 115:67. PubMed
- Obata-Ninomiya K, et al. 2013. J Exp Med. 210:2583. PubMed
- Shmuel-Galia L, et al. 2016. EMBO J. 35: 685 - 698. PubMed
- Iwata A, et al. 2014. Int Immunol. 26:103. PubMed
- Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
- Mukovozov I, et al. 2015. J Immunol. 195: 3334 - 3344. PubMed
- RRID
- AB_2028401 (BioLegend Cat. No. 135507) AB_2085223 (BioLegend Cat. No. 135508)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Macrophage colony stimulating factor (M-CSF), IL-34
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Sudo T, et al. 1995 Oncogene 11:2469.
- Murayama T, et al. 1999 Circulation 99:1740.
- Goswami S, et al. 2005 Cancer Res. 65:5278.
- Yu W, et al. 2008 J. Leuko. Biol. 84:852.
- Gene ID
- 12978 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org
