Alexa Fluor® 488 anti-mouse CD115 (CSF-1R) Ant
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD115 (CSF-1R) Ant
- 产品类别：
- 抗体
- 产品编号：
- 135511
- 产品应用：
- 135511
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.?Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.
- Application References
(PubMed link indicates BioLegend citation) -
- Sudo T, et al. 1995. Oncogene 11:2469.
- Murayama T, et al. 1999. Circulation 99:1740.
- Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
- Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
- Dong L, et al. 2016. Nature. 539:304–308. PubMed
- Product Citations
-
- Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
- Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
- Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
- Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
- Ghaffari S, et al. 2020. Arterioscler Thromb Vasc Biol. ATVBAHA120314557:. PubMed
- Sharif S, et al. 2016. PLoS One. 11:e0168007. PubMed
- Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
- Kuboi Y, et al. 2019. Int Immunol. 31:287. PubMed
- Bachy S, et al. 2022. iScience. 25:103758. PubMed
- Halder LD, et al. 2022. MBio. :e0356321. PubMed
- Halder LD, et al. 2020. Nat Commun. 2.077083333. PubMed
- RRID
- AB_11218605 (BioLegend Cat. No. 135511) AB_11218983 (BioLegend Cat. No. 135512)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Macrophage colony stimulating factor (M-CSF), IL-34
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Sudo T, et al. 1995 Oncogene 11:2469.
- Murayama T, et al. 1999 Circulation 99:1740.
- Goswami S, et al. 2005 Cancer Res. 65:5278.
- Yu W, et al. 2008 J. Leuko. Biol. 84:852.
- Gene ID
- 12978 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org
