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首页 > 抗体蛋白 > 抗体
Purified anti-mouse H-2K
产品名称：
Purified anti-mouse H-2K
产品类别：
抗体
产品编号：
116501
产品应用：
116501
[价格]
规格 价格 库存
50µg ￥ 924 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).

Application References

(PubMed link indicates BioLegend citation)
  1. Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
  2. Wall KA, et al. 1983. J. Immunol. 131:1056.
  3. Andersson M, et al. 1998. J. Immunol. 161:6475.
  4. Shao H, et al. 2005. J. Immunol. 175:1851.
  5. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
  6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
  7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
Product Citations
  1. Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed
  2. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  3. Robert S, et al. 2018. Bone Marrow Transplant. 53:807. PubMed
  4. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  5. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  6. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  7. Stefanski HE, et al. 2018. PLoS One. 13:e0198871. PubMed
  8. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  9. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
  10. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
  11. Shao H, et al. 2005. J Immunol. 175:1851. PubMed
RRID
AB_313728 (BioLegend Cat. No. 116501) AB_313729 (BioLegend Cat. No. 116502)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb on UniProt.org

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