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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse H-2K
产品名称：
Pacific Blue™ anti-mouse H-2K
产品类别：
抗体
产品编号：
116513
产品应用：
116513
[价格]
规格 价格 库存
25µg ￥ 1344 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).

Application References

(PubMed link indicates BioLegend citation)
  1. Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
  2. Wall KA, et al. 1983. J. Immunol. 131:1056.
  3. Andersson M, et al. 1998. J. Immunol. 161:6475.
  4. Shao H, et al. 2005. J. Immunol. 175:1851.
  5. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
  6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
  7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  3. Dong L, et al. 2021. Cancer Cell. . PubMed
  4. Bistoni G, et al. 2012. Biomaterials. 33:4762. PubMed
  5. Gurusamy D, et al. 2020. Cancer Cell. 37(6):818-833.e9. PubMed
  6. Capuccini B, et al. 2016. Sci Rep. 6:39258. PubMed
  7. Allen L 2004. J Immunol. 173:835. PubMed
  8. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
RRID
AB_1967103 (BioLegend Cat. No. 116513) AB_1967129 (BioLegend Cat. No. 116514)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb on UniProt.org

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