Brilliant Violet 510™ anti-mouse H-2K-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 2940	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C57BL mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation² and immunohistochemical staining of acetone-fixed frozen sections³. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).

Application References (PubMed link indicates BioLegend citation)

Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
Wall KA, et al. 1983. J. Immunol. 131:1056.
Andersson M, et al. 1998. J. Immunol. 161:6475.
Shao H, et al. 2005. J. Immunol. 175:1851.
Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed

RRID

AB_2800584 (BioLegend Cat. No. 116523)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972 View all products for this Gene ID
UniProt: View information about H-2Kb on UniProt.org

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