Alexa Fluor® 700 anti-mouse H-2K-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1272	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C57BL mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation² and immunohistochemical staining of acetone-fixed frozen sections³. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).

Application References (PubMed link indicates BioLegend citation)

Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
Wall KA, et al. 1983. J. Immunol. 131:1056.
Andersson M, et al. 1998. J. Immunol. 161:6475.
Shao H, et al. 2005. J. Immunol. 175:1851.
Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed

Product Citations

Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
Goddery EN, et al. 2021. Front Immunol. 12:726421. PubMed

RRID

AB_2750392 (BioLegend Cat. No. 116521) AB_2750393 (BioLegend Cat. No. 116522)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972 View all products for this Gene ID
UniProt: View information about H-2Kb on UniProt.org

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