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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse I-A
产品名称：
PerCP/Cyanine5.5 anti-mouse I-A
产品类别：
抗体
产品编号：
116415
产品应用：
116415
[价格]
规格 价格 库存
25µg ￥ 1692 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10J splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Does not react with other haplotypes (e.g., d, f, q, r, s).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
  2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
  3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
  4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
  5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
  6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
  7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
  8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
  9. Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
Product Citations
  1. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  2. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  3. Wong E, et al. 2019. Cell Rep. 29:3047. PubMed
  4. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  5. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  6. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  7. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  8. Uto-Konomi A, et al. 2012. PLoS One. 7:e40343. PubMed
  9. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  10. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  11. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  12. Yá?ez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_1953308 (BioLegend Cat. No. 116415) AB_1953309 (BioLegend Cat. No. 116416)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ab on UniProt.org

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